Doxycycline抑制大肠癌LS174T细胞侵袭的体外实验研究

Effects of Doxycycline on Invasion and Metastasis of Colorectal Cancer Cell Line LS174T

目的:观察多西环素(Doxycycline)在体外对LS174T侵袭能力的抑制作用,并尝试探讨该抑制作用的分子机制。方法:1)MTT法观察Doxycycline对LS174T细胞粘附力的影响;Transwell小室法检测不同浓度Doxycycline对细胞侵袭和运动能力的抑制作用;2)RT-PCR法检测不同浓度Doxycycline作用48h后,MMP2的mRNA表达情况,同时明胶酶谱法检测MMP2分泌。结果:1)Doxycycline能够以剂量依赖方式抑制大肠癌LS174T细胞的体外运动和侵袭能力,但不影响其粘附能力;2)Doxycycline能够抑制LS174T细胞MMP2的mRNA表达。结论:Doxycycline在体外能够有效地抑制大肠癌LS174T细胞的侵袭、转移,其中MMP2是抑制大肠癌细胞LS174T的一个有效靶点。

Objective： To observe the effects of Doxycycline on matrix metalloproteinase-2 （MMP2） mRNA expression, invasion, and biological activities in the human colorectal cancer cell line LS174T in order to shed light on the molecular mechanism behind Doxycycline＇s ability to inhibit tumor invasion and metastasis. Methods： a） The effect of Doxycycline on the adhesion activity of LS174T was observed using MTT reduction assay （M3T）, and the inhibitory effects on invasion and motility were detected using the Trans-well zeta method, b） RT-PCR was used to detect the mRNA expression of MMP2. At the same, total protein was extracted and used to detect MMP2＇s gelatinase activity. Results： a） Doxycycline could inhibit the in vitro motility and invasion abilities of the LS174T cells in a dose- dependent manner, with no effect on adhesion, b） Doxycycline could also inhibit the expression of MMP2 mRNA. Conclusion： Doxycycline can effectively inhibit the growth, invasion and metastasis of the LS174T colorectal cancer cells in vitro by inhibiting mRNA expression of MMP2.