摘要
目的:探讨猕猴大脑中动脉阻塞/再灌(MCAO)后海马CA1区胰岛素样生长因子-1(IGF-1)的表达。方法:采用猕猴MCAO模型,缺血2h,再灌24h,用免疫组化方法检测脑缺血后IGF-1蛋白的表达。结果:MCAO后缺血侧海马CA1区形态结构改变,神经元密度降低,IGF-1蛋白阳性细胞数与假手术组比较明显减少(P<0.05)。结论:MCAO缺血侧海马CA1区结构改变,IGF-1蛋白表达减少,提示海马内IGF-1在脑缺血损伤中可能具有一定的作用。
Objective：To explore the expression of insulin-like growth factor-1 （IGF-1） following the middle cerebral artery occlusion/reperfusion （MCAO） in monkeys.Methods：The immnuohistochemistry was applied to detect the expression of IGF-1 protein following 2 h MCAO and 24 h reperfusion in 13 young monkeys either of sex.Results：Structural changes and neuron loss were observed in hippocampus CA1 region. The number of immuno-positive neurons were decreased after ischemia this region following MCAO （P〈0.05）.Conclusion：The neuron loss and decreased expression of IGF-1 in hippocampus CA1 suggested that IGF-1 might play a role on cerebral ischemia.
出处
《中国医药导报》
CAS
2007年第06S期19-20,共2页
China Medical Herald
基金
青岛市科技局基金项目(编号:No.2004-wszd024)
