血液乙醇检测内外标法比较研究

A comparative study of the internal and external standard methods in the detection of alcohol levels in blood samples

目的通过比较内标法和外标法对血液乙醇含量检测结果，探讨外标法在法医学实践中的应用价值。方法通过收集2005年10月-2006年12月间本中心符合检测要求的血液样品263例，每例分别采用内标法和外标法进行血液样品乙醇含量检测，比较其检测结果。结果外标法检测时间短（2．5min），用量0．5ml，而内标法检测时间长（6．5min），样品量1．0ml；外标法检测血乙醇平均浓度为89．30mg／100ml，内标法检测血乙醇平均浓度为92．37mg／100ml，P=0．001。结论外标法检测时间短（2．5min），用量少，节约检材，可作为大量待测血液样本的筛选检查手段。

This article attempts to demonstrate the potential benefits of employing external standard methodin forensic applications by comparing the results of using internal standard method and the external standard method in the detection of alcohol levels in blood samples. Method Detecting the alcohol levels in 263 qualified blood samples collected from October 2005 to December 2006 by applying both external standard methodand internal standard method to each sample. Results The external standard method took shorter time （2.5 minutes） and less volume （0.5ml） compared to 6.5 minutes and 1.0ml for internal standard meth od. The average blood alcohol density detected by external standard method was 89.30mg/100ml versus 92.37mg/100ml by internal standard method, p=0. 001. Conclusion The external standard method is the preferred filtering method for large scale blood samples in alcohol detection because it is faster, uses less blood volume and saves testing materials.