HPLC测定蒙药五味沙棘散中栀子苷含量

Determination of the Content of Geniposide in Mongolian Medicine Wuwei Shaji San by HPLC

目的:建立蒙药五昧沙棘散中栀子苷含量测定方法。方法:采用HPLC,使用C_(18)柱(150mm×4．6mm, 5μm),流动相为乙腈-0．1%磷酸水溶液(15:85);检测波长为238nm。结果:栀子苷在0．135～0．690μg线性关系良好,栀子苷平均回收率为98．87%,RSD=1．03%。结论:该方法简便易行,结果准确可靠,可用于蒙药五味沙棘散含量测定。

Objective： To establish a determination of geniposide in Mongolian medicine Wuwei Shaji San by HPLC, Methods： HPLC with C18 （150mm×4. 6mm, 5μm） column was used. The flow phase is acetonitrile - 0. 1% phosphoric acid （15：85 ） and the measured wavelength is 238nm. Results： The linear range was from 0. 135μg to 0. 690μg for geniposide. The.average recovery of geniposide was 98.87 %, RSD = 1.03%. Conclusion： The motheds are simple. The result is accurate and reliable. The methods can be used as the quality control of Mongolian medicine Wuwei Shaji San.