摘要
目的:建立头孢特仑新戊酯片含量测定的高效液相色谱方法。方法:采用岛津CLASS VP C18(250mm×4.6mm,5μm)色谱柱;以乙腈:醋酸钠缓冲液(35:65)为流动相;以对羟基苯甲酸甲酯为内标;检测波长254nm;调整流量使头孢特仑新戊酯峰的保留时间为14min。结果:头孢特仑新戊酯的线性范围为100~1000μg·mL^-1,r=0.9999,平均回收率为99.63%,RSD为0.57%。结论:本法可用于头孢特仑新戊酯片的含量测定,操作简便,结果准确。
Objective:To establish a HPLC method for the determination of cefteram pivoxil tablets. Method: Shimadzu CLASS VP C18 column (250 mm × 4.6 mm, 5μm) was selected, with a mobile phase consists of ace-tonitrile -0.05 mol·L^-1 sodium acetate buffer solution (35: 65 ), at a detection wavelength as 254 nm. The in-ternal standard was methyl p-hydroxybenzoate, and the flow rate was adjusted to make the retention time of cefteram pivoxil about 14 minute. Results: The calibration curve was proved to be linear in the range of 100~1000μg·mL^-1(r=0. 9999). The average recovery was 99.63% (RSD=0.57% ). Conclusion: The proposed method is simple, sensitive and accurate.
出处
《药学与临床研究》
2007年第3期210-212,共3页
Pharmaceutical and Clinical Research