归芍合剂中丹参的鉴别和芍药苷的含量测定

Identification of Danshen and content determination of paeoniflorin in Guishao mixture

目的:归芍合剂由白芍、丹参等10味中药组成,芍药苷为其有效成分之一,通过实验制定归芍合剂中芍药苷的含量测定方法。方法:薄层色谱法鉴别丹参:以丹参素钠为对照品,以氯仿-丙酮-甲酸(25∶10∶4)为展开剂;HPLC法测定芍药苷的含量:Spherisorb C18柱(4.6 mm×300 mm,5μm),流动相:甲醇-水-磷酸(28∶72∶0.1),流速:1.0 mL.min-1,检测波长230 nm,柱温:20℃。结果:芍药苷的平均回收率为98.0%,RSD为1.84%。结论:本实验方法简便,准确,重现性好。

Objective： To improve the quality standard of Guishao mixture. Methods： TLC and HPLC were used for the idenification of Danshen and HPLC was used for the determination of paeoniflorin. Results ：The average recovery of the main active ingredient paeoniflorin is 98.0% with RSD 1.83%. Conclusions： These two items involved in the method can improve the quality control of Guishao mixture.