血视神经屏障特性的研究

A comparative study of blood-optic nerve barrier properties

目的:研究血视神经屏障与血脑屏障之间的差异。方法:SD雄性大鼠20只,分别取筛板前区、筛板区、筛板后区、眶内段、管内段、颅内段和大脑皮层。用电镜观察各部位微血管内皮细胞的超微结构;用免疫组织化学的方法检测转铁蛋白受体(OX-26)的表达及微血管周围纤维蛋白原(fibrinogen)外渗情况。结果:筛板前区质膜囊泡较大脑皮层多(P<0.05)。筛板前区微血管内皮细胞OX-26表达阴性。微血管周围fibrinogen阳性。结论:筛板前区微血管内皮细胞不具有血脑屏障特性。

Objective ：To investigate the difference of endothelial cell in ultrastructure,markers and permeability between blood-brain barrier and blood-optic nerve barrier. Methods ：Twenty normal male SD rats＇ optic nerve （prelaminar region,lamina cribrosa, retro-laminar region,intraorbital portion,intracanalicular portion, intracranial portion） and cerebral cortex were removed separately. Uhrastructure of endothelial cell was observed by electron microscopy. Employing staining the relative distribution was determined for the OX-26 and extravasated fibrinogen around microvessels. Results： Prelaminar region had a significantly higher number of plasmalemmal vesicles than that of cerebral cortex （P 〈 0.05 ） ; Microvessels in the prelaminar region showed no expression of the OX-26. but fibrinogen was positive around microvessels. Conclusions： Microvessels in prelaminar region lack the typical blood-brain barrier characteristics.