纳洛酮对缺氧-复氧心肌细胞线粒体能量代谢的保护作用

Effect of Naloxone on delay of the collapse of mitochondrial membrane potential and improvement of energy metabolism of cultured myocardial cell induced by hypoxia and reoxygenation

目的:观察纳洛酮对培养家兔心室肌细胞缺氧-复氧后线粒体膜电位变化的影响,探讨其对缺氧心肌细胞能量代谢的保护意义。方法:培养心室肌细胞分成三组:对照组(A组)、缺氧组(B组)、纳络酮组(C组),后两组又分3个亚组:缺氧2 h组(B1、C1);缺氧2 h-复氧2 h组(B2、C2);缺氧2 h-复氧4 h组(B3、C3)。检测缺氧-复氧后不同时间细胞线粒体膜电位变化情况。结果:缺氧后,线粒体膜电位均下降;C组各时间点线粒体膜电位均高于B组(P≤0.01)。结论:早期应用纳洛酮可明显减轻心室肌细胞缺氧-复氧后线粒体膜电位的下降,改善缺氧心肌细胞的能量代谢。

Objective ：To study the effect of Nalonxe on mitochondrial membrane potential（MMP） in cultured myocardial cell after hypoxia-reoxygenation and its possible protection to cardialmyocytes＇energy metabolism. Methods：cultured cardiomyocytes from neonate rabbits were divided into three groups（ control group, hypoxia group and naloxone group） and the two latter were further divided into three subsections： hypoxia 2 hour, hypoxia 2 h-reoxygen 2 h, hypoxia 2 h-reoxygen 4 h. Then the ceils were stained with fluorescent dyes and MMP was assayed by flow cytometry. Results：After hypoxia, MMP of hypoxia group and Naloxone group were lower than the eontrol（P≤0.01 ）. MMP of therapy group was significantly higher than that of hypoxia group on each different time point（P≤0.01 ）. Conclusion：Naloxone might significantly reduce the lossing of MMP of hypoxia-reoxygenation injured cardialmyocytes and improve their energy metabolism.