乌司他丁对肠源性脓毒症大鼠D-二聚体和蛋白C系统的影响

Effects of ulinastatin on serum D-dimer and protein C system in rats with severe gut-origin sepsis

目的:观察乌司他丁(ulinastatin,UTI)对肠源性脓毒症大鼠D-二聚体(DD)、蛋白C(PS)系统的影响。方法:将Wistar大鼠随机分为4组,假手术组(SH组,n=36)、脓毒症组(SEP组,n=30)、UTI 3h给药组(UTI3h组,n=30)和12h给药组(UTI12h组,n=18)。利用经典盲肠结扎穿孔法建立肠源性脓毒症大鼠模型,分别于正常对照组及制模后6、12、24、48及72h组心脏取血测定DD、PC和蛋白S(PS)。结果:与SH组相比,SEP组大鼠DD在造模后逐渐升高,PC、PS含量降低,差异有显著性(P<0.05)。与SEP组相比,UTI3h、UTI12h组大鼠的DD降低,PC含量下降趋势减弱,差异有显著性(P<0.05)。结论:UTI能有效改善脓毒症期间的抗凝抑制和继发性纤溶亢进。

Objective To observe the effects of ulinastatin （UTI） on serum D-dimer （D-D） and protein C （PC） system in rats with severe gut-origin sepsis. Methods One hundred and fourteen Wistar rats were randomly divided into four groups： sham group （n=36）, sepsis group （n=30）, UTI3h group （n=30）, and UTI12h group （n=18）. The rat model of gutorigin sepsis was established by classic cecal ligation and puncture （CLP）. Serum levels of D-D, PC, and protein S（PS） were detected in the normal and experimental rats 6, 12, 24, 48, and 72 h after CLP. Results The D-D level was higher but the levels of PC and PS were significantly lower in the sepsis group than in the sham group （P 〈 0.05）. As compared with those in the sepsis group, D-D was decreased and PC was increased markedly in the UTI3h or UTI12h group （P 〈 0.05）. Conclusion Ulinastatin can effectively improve the suppression of anticoagulation and resultant excessive fibrinolysis.