白细胞介素18在喉鳞状细胞癌中的表达

Expression of interleukin-18 in laryngeal squamous cell carcinoma

目的探讨白细胞介素18(interleukin-18,IL-18)在喉癌发生发展中的作用。方法采用双抗体夹心ELISA法检测22例喉癌患者(喉癌组)和10例健康人(对照组)血清中IL-18的含量;采用RT-PCR法检测22例配对喉癌组织及切缘黏膜组织中IL-18基因的表达。结果喉癌组血清IL-18含量(68.44±39.42)pg/ml高于对照组(28.44±2.29)pg/ml,差异有显著性(P<0.01);RT-PCR检测IL-18mRNA,22例喉癌组织均无表达;癌旁黏膜IL-18mRNA有9例(40.9%)表达。结论喉癌患者血清IL-18表达增高,提示IL-18与喉癌的发生发展相关;喉癌组织IL-18mRNA不表达,而癌旁黏膜部分表达,表明IL-18并非肿瘤细胞所分泌,可能来自宿主细胞。

OBJECTIVE To investigate the role of IL-18（Interleukin-18） in the pathogenesis of laryngeal carcinoma. METHODS The IL-18 concentration in sere of 22 laryngeal carcinoma patients and 10 health control subjects were measured with enzyme-linked immunosorbent assay （ELISA）. The gene expression of IL-18 in fresh laryngeal cancer tissues and their surrounding mucosa was analyzed by RT-PCR in the same patient. RESULTS The serum concentration of IL-18 in patients with laryngeal carcinoma （68.44± 39.42）pg/ml was significantly higher compared with that in health control subjects （28.47±2.29）pg/ml （P〈0.01）. RT-PCR analysis of the cancer tissues exhibited no IL-18 mRNA expression, but 9 cases（40.9 %） showed IL-18 mRNA expression in their surrounding mucosa. CONCLUSION In this study, IL-18 serum levels were significantly enhanced in patients with laryngeal cancer than that in healthy controls. These results suggest that overproduction of IL-18 may be induced in response to tumor cells or other factors related to tumor growth. As RT-PCR analysis of cancer tissue exhibited no IL-18 expression, and only part of their surrounding mucosa showed expression of IL-18, thus the IL-18 may originate from tumor-infiltrating host cells, not from tumor cells.