健康人鼻咽部表面活性物质的测定

Measurement of the surfactant in irrigating fluid from nasopharynx in healthy persons

目的探讨健康人鼻咽部表面活性物质的状况,并对磷脂成分进行研究。方法采集健康人鼻咽部灌洗液,用高效液相色谱方法对表面活性物质的磷脂酰丝氨酸、磷脂酰乙胺醇、磷脂酰胆碱和鞘磷脂进行测定。结果①鼻咽部存在表面活性物质,磷脂4种成分均可测出;②磷脂成分的构成中,磷脂酰胆碱含量最多,为(53.94±17.10)%,是主要成分,其次是鞘磷脂和磷脂酰丝氨酸,分别是(22.18±24.08)%和(17.58±9.03)%,磷脂酰乙醇胺只占小部分,为(6.30±4.28)%。结论①鼻咽部存在表面活性物质;②鼻咽部表面活性物质的磷脂由磷脂酰丝氨酸、磷脂酰乙胺醇、磷脂酰胆碱和鞘磷脂组成,磷脂酰胆碱含量最多,对鼻咽部表面活性物质起决定作用。

OBJECTIVE To investigate the situation of nasopharyngeal surfactant in healthy persons, and to study biochemical component of phospholipid of surface active substance. METHODS The concentrations of surfactant in nasopharyngeal irrigating fluid were implemented in healthy persons. Component of phospholipid such as phosphatidylsedne, phosphatidyl ethanolamine, phosphatidylcholine and sphingophospholipid were measured by the highperformance liquid chromatograph. RESULTS （1） There was surfactant in nasopharynx. Four compositions of phospholipid could be measured; （2） In composition of phospholipid, the percentages of phosphatidylcholine , sphingophospholipid, phosphatidylserine and phosphatidyl ethanolamine were （53.94±17.10） %, （22.18±24.08） %, （17.58±9.03） % and （6.30 ±4.28） % respectively. CONCLUSION （1） There is surfactant in nasopharynx; （2）Nasopharyngeal surfactant is made of phosphatidylcholine, sphingophospholipid, phosphatidylserine and phosphatidyl ethanolamine.