海藻糖合酶产生菌基因组DNA的提取方法

Rapid extracting approach of the genomio DNA from producing trehalose synhase strain

以海藻糖合酶产生菌一恶臭假单胞菌为目标菌,建立了一种快速、简便提取假单胞菌染色体DNA的方法,利用本方法提取的KNA结构完整、无降解、纯度较高,无需纯化,可直接作为PCR方法扩增目的基因的模板。

A rapid and simple approach in the genomic DNA extracting from the pseudomonas putida 1. 1819 which can produce trehalose synthase was promoted in this paper. This method was both easier and economical. Results showed that DNA isolated with this method was intergrated and purely. The purity of the obtained DNA was good enough for the PCR analysis experiment.