用快速酶联免疫法检测放射工作人员巨细胞病毒感染

Detection of HCMV Infection of Radiation Workers with Rapid-ELISA Method

目的建立了一种快速检测人巨细胞病毒(HCMV)抗体的酶联免疫方法,了解放射工作人员HCMV感染状况。方法应用常规ELISA法稀释液中加入3%聚乙二醇(PEG)来加速抗原抗体反应的快速法,对放射工作人员血清HCMV-IgG、HCMV-IgM进行检测。结果514例济南地区放射工作人员中总阳性率为96.69%,其中94.16%为潜伏感染,0.19%为原发感染,2.34%为复发感染,活动性感染率为2.53%。结论放射工作人员患HCMV感染性疾病存在一定的危险性,应引起高度重视。

Objective To establish a rapid method to detect HCMV with ELISA, and to study the infection conditions of radiation workers. Methods using routine ELISA except adding 3% PEG to dilution solution to accelerate the reaction of antibody to antigen, the HCMV - IgG and HCMV - IgM in the serum of radiation workers were detected. Results The total positive rate in 514 ratiation workers in Jinan is 96.69% , of which 94.16% is latent infection, 0.19% is primary infection, 2.34% is relapse infection, and active infection rate is 2.53%. Conclusion Radiation workers have certain risk to suffer from infection of HCMV which should delt with seriousty.