分次剂量可变方案照射MCF-7乳腺癌细胞的实验研究

Research on the Effect of Fractionated Irradiation with Variable Fraction Sizes with SMMC7721 Liver Malignancy Cells

目的用人乳腺癌细胞MCF-7研究不等分次剂量(VFS)照射方案的效果。方法10组相同数目的MCF-7细胞样本,用MTT分析方法获得不同的分次剂量方案照射后的细胞存活率(S)进行比较。结果除1组外,其余9组VFS方案照射后获得的S值均小于CFS照射后的S值,其中有统计学意义的有3组;在相同变化幅度的情况下,递减的VFS照射方案所获得的效果要好于递增VFS照射方案,其中有显著的统计学意义的有2组。结论实验结果与理论计算结果一致,所以,VFS方案,尤其是分次剂量递减的VFS方案可能应用于临床以提高治疗效果。

Objective To analyze the effect of fraetionated irradiation with variable fraction sizes （VFS） with human mammary tumor cells MCF - 7. Methods Ten samples with the same number of MCF - 7 cells were irradiated with different fractionated protocols, then using MTT analysis method, the cell survival probability（S） is obtained and compared with each other. Results In nine groups, the S value of the samples irradiated with VFS protocols are lower than that of the sample irradiated with constant fraction sizes （CFS） protocol, also the differences between some of the results are of statistical significance; And for the same variety range, the effect of irradiation with decreasing fraction sizes is better than that of irradiation with increasing fraction sizes, also the differences of S value between two groups are of statistical significance. Condusion The result of experiment with MCF - 7 cells conformes to the former calculation results. Therefore the VFS irradiation protocol, especially the protocol with decreasing fraction sizes can probalely be used in clinics to enhance the treatment effect.