睾酮对高脂饲养小鼠主动脉超微结构的影响

Effect of Testosterone on Artery Pathology in Cholesterol-fed Male Mice

目的研究睾酮对高脂刺激的雄性小鼠主动脉超微结构的影响。方法56只小鼠随机分为4组,其中1、2、3组给去势术,4组行假手术。2组(小剂量补充睾酮组)、3组(大剂量补充睾酮组)分别给予10mg.kg-1.d-1、15mg.kg-1.d-1的睾酮腹腔注射;1组(低睾酮组)、4组(正常对照组)给予安慰剂。各组小鼠均高脂饲养,3个月后测定部分血小板、血脂、凝血指标,并电镜观察主动脉的超微组织结构变化。结果1组、3组光镜及电镜下均呈早期动脉粥样硬化(AS)改变,1组与2组、4组相比,LDL-C、PAI明显升高,HDL-C下降,P<0.05;各组之间血小板计数、血小板聚集率、TCH、TG差异均无显著性,P>0.05。结论内源性睾酮有防止AS的作用,适量补充外源性睾酮可以替代内源性睾酮的作用,低血清睾酮及大量补充睾酮均促进AS的发生。

Objective To study the effect of endogenic and ectogenic testosterone（T） on artery pathology in cholesterol - fed male mice. Methods 56 mice were divided randomly into four groups. Group 1 was castrated only （hypotestosterone,n= 14）,group 2 （n= 14） and group3 （n= 14） were given respectively 10mg·kg^-1·d^-1,15mg. kg·kg^-1·d^-1 T through abdominal cavity injection after castration, placebo were given to group 4 （n = 14, sham castration）and groupl. All rats were fed with cholesterol- rich diet during 12 - week treatment period, When the test was over, blood samples were obtained for detection of platelet count, platelet aggregation rate, TC,HDL-C,TG,LDL-C,PAI,then mice were killed and thorax arteries were taken out for examinations of pathology by optical and electronic microscope. Results Early period AS appeared in arteries of group 1 and 3. Compared with group 2 and 4, both LDL - C and PAl in group 1 were obviously higher, while HDL- C was significantly decreased（P〈0, 05）, PAl was higher in group 3 than in group 4 （P 〈 0.05）. No deference was ob- served in each group in platelet count, plateletaggregation rate, TC and TG, Conclusion Endogenic T plays a role in inhibiting aortic AS in male mice,supplying proper dose ectogenic T can take place of the role of endogenic T, Hypotestosterone and high dose T can expedite the development of AS.