复方麦冬丸的镇痛抗炎作用

Analgesic and anti-inflammatory effect of compound Maidongwan

目的:观察复方麦冬丸的镇痛抗炎作用。方法:实验于2006-07/10在三峡大学医学实验中心完成。昆明种小鼠240只,雄性SD大鼠120只。各实验中的受试动物均随机分为6组,每组10只。①小鼠实验所分6组为生理盐水组、阿司匹林200mg/kg组、金嗓子喉宝1800mg/kg组和复方麦冬丸1350,1800,2250mg/kg组。采用冰醋酸致小鼠扭体反应、热板致痛的方法观察复方麦冬丸的镇痛作用。使用二甲苯致小鼠耳肿胀实验、醋酸引起小鼠腹腔毛细血管通透性增高实验观察复方麦冬丸的抗炎效应。②大鼠实验所分6组为生理盐水组、阿司匹林150mg/kg组、金嗓子喉宝1250mg/kg组、复方麦冬丸937.5,1250,1562.5mg/kg组,慢性肉芽肿实验中以氢化可的松代替阿司匹林。采用蛋清诱发大鼠足肿胀、棉球诱发大鼠肉芽肿的方法及测定蛋清致炎大鼠血清中超氧化物歧化酶活性、丙二醛含量、一氧化氮含量、一氧化氮合酶活性、大鼠炎性组织中前列腺素E2含量观察复方麦冬丸的抗炎效应。结果:①复方麦冬丸3个剂量组与生理盐水组比较均能显著减少冰醋酸诱发的小鼠扭体次数,且3个剂量组之间呈现出扭体次数由低到高逐渐下降的趋势。复方麦冬丸3个剂量组的作用均强于金嗓子喉宝组,但仍不及阿司匹林组。②金嗓子喉宝组可显著抑制热板引起的疼痛。阿司匹林和复方麦冬丸对热板引起的疼痛无显著抑制作用。③复方麦冬丸3个剂量组与生理盐水组比较均能显著缓解二甲苯所致的小鼠耳肿胀度,其作用与金嗓子喉宝大体相当但比阿司匹林略差。④复方麦冬丸对醋酸引起小鼠腹腔毛细血管通透性增高有显著抑制作用,其作用与金嗓子喉宝大体相当但远不如阿司匹林。⑤复方麦冬丸对蛋清诱发大鼠足肿胀有显著抑制作用。⑥复方麦冬丸对棉球诱发肉芽肿有显著抑制作用。⑦复方麦冬丸3个剂量组与生理盐水组和金嗓子喉宝1250mg/kg组比较均能显著抑制前列腺素E2,复方麦冬丸937mg/kg组与1250mg/kg组抑制前列腺素E2的作用甚至强于阿司匹林。⑧复方麦冬丸3个剂量组与生理盐水组和阿司匹林组分别比较均明显升高超氧化物歧化酶活性,降低丙二醛含量(均为P<0.01)。复方麦冬丸1562mg/kg组与其他组比较能降低一氧化氮含量、一氧化氮合酶活性(P<0.01)。结论:复方麦冬丸可能是一个弱镇痛药,但抗炎作用显著。其抗炎机制可能与升高超氧化物歧化酶活性,降低丙二醛含量、一氧化氮含量、一氧化氮合酶活性和炎性组织中前列腺素E2含量有关。除扭体实验中复方麦冬丸表现出量效关系,其余实验并未表现出明显的剂量关系。

AIM： To observe the analgesic and anti-inflammatory effect of compound Maidongwan. METHODS： The experiment was conducted in the Medical Experimental Center, Laboratory of Three Gorges University from July to October 2006. 240 Kunming mice and 120 male SD rats ware selected and randomly divided into 6 groups with 10 animals in each group. （1） Mice were divided into 6 groups： Normal saline group, 200 mg/kg aspirin group, 1 800 mg/kg Jinsangzihoubao group as well as 1 350 mg/kg, 1 800 mg/ kg, and 2 250 mg/kg Maidongwan groups. The writhing response was provoked by glacial acetic acid in mice and hot plate method was adopted to observe analgesic effects of compound Maidongwan. Rat models of ear-swelling was induced by dimethyl benzene, and the anti-inflammatory effect of compound Maidongwan was observed by enhancing the permeability of enterocoelia capillary in mice with acetic acid. （2） Rats were randomly divided into 6 groups： Normal saline group, 150 mg/kg aspirin group, 1 250 mg/kg Jinsangzihoubao group as wall as 937.5 mg/kg,1 250 mg/kg, and 1 562.5 mg/kg Maidongwan group. Cetacort was used instead of aspirin in chronic grenuloma experiment, and rat forced foot was induced with albumen. Rat granulomatous formation was caused by tampon. The levels of serum superoxide dismutase （SOD）, malonaldehyde （MDA）, nitrogen monoxidum （NO）, nitric oxide synthase （NOS） and the prostaglandin E2 （PGE2） content in inflammatory tissues of rats ware determined to observe the anti-inflammatory effects of compound Maidongwan. RESULTS： （1） The writhing response induced by glacial acetic acid was significantly reduced in compound Maidongwan groups of 3 doses and the normal saline group. Besides, the writhing response and was in a negative correlation with the dose of compound Maidongwan, while the effect in compound Maidong wan was better than that in the Jinsangzihoubao group, but a little worse than that in aspirin group. （2） Jinsangzihoubao could significantly inhibit the pain induced by hot plate, while there was no remarkable effect of aspirin and Maidongwan. （3） Compared with the normal saline group, Maidongwan could remarkably ameliorate the ear swelling provoked by dimethyl benzene at 3 doses, and the effect was similar to that of Jinsangzihoubao, but a little weaker than that of aspirin. （4） Compound Maidongwan could significantly restrain the permeability of enterocoelia capillary in mice raised by acetic acid, and the effect was similar to Jinsangzihoubao but far away from aspirin. （5） Compound Maidongwan had remarkable depressant effect on rat forced foot induced by albumen. （6） Rat granulomatous formation caused by tampon was remarkably inhibited by compound Maidongwan. （7） Compound Maidongwan groups of 3 doses had better effects in inhibiting PGE2 content than that of normal saline group and 1 250 mg/kg Jinsangzihoubao group. Moreover, the effects of 937 mg/kg and 1 250 mg/kg Maidongwan groups were better than that of aspirin group, which indicated that compound Maidongwan could remarkably inhibit pGE2. （8） SOD activity and MDA content in 3 Maidongwan groups ware significantly higher than those in normal saline group and aspidn group （P〈 0.01）. NO content and NOS activity in 1 562 mg/kg compound Maidongwan group were significantly lower than those in other groups （P 〈 0.01）. CONCLUSION： Compound Maidongwan might be a weak pain-killer, but its anti-inflammatory effect is remarkable. The anti-inflammatory mechanism of compound Maidongwan may be due to that it can enhance SOD activity and reduce the levels of MDA, NO, NOS and PGE2. There is a dose-dependent effect in writhing response experiment, but none is found in other experiments.