胰岛移植过程中胰岛培养的研究现状

Development of islet culture in islet transplantation

目的:总结胰岛移植过程中胰岛培养的研究现状,为利用胰岛培养准备更多更好的胰岛提供理论依据和技术支持。资料来源:以计算机检索Medline和Pubmed数据库1994-01/2007-01与胰岛培养相关的文章,检索词为“isle,tculture”,限定文章语言种类为English。同时计算机检索CNKI中国全文数据库1994-01/2007-01与胰岛培养相关的文章,检索词为“胰岛,培养”,限定文章语言种类为中文。资料选择:对资料进行初审,纳入标准:①与胰岛培养相关的文章。②1994年以后发表的文章。排除标准:Meta分析类文章。资料提炼:共检索到1384篇与胰岛培养相关的文章,入选33篇。对所检索文章的相关信息加以综合概括,其中关于胰岛培养中基础培养基、培养添加物、培养温度、密度等方面的文章18篇,关于胰岛培养新尝试的文章12篇。资料综合:将胰岛培养后再行移植有一定优势,包括降低免疫原性和为术前准备赢得时间等。目前对于挑选合适培养基及其添加物,调整合适培养温度、培养密度以及保护胰岛的活性等都有了比较深入细致的研究;同时很多学者开始尝试一些新的方法延长胰岛培养时间,改善胰岛功能,包括加入细胞外基质、与其他细胞共培养、选用微重力系统培养以及利用胶囊包裹培养等均显示良好的效果。结论:虽然胰岛培养可能会影响胰岛的活性和功能,但随着对胰岛生理和病理生理特性研究的深入,新的技术方法可将这些影响降到最低。胰岛培养给临床实践带来的便利是其最大的优势,加强胰岛培养的研究与应用有着重要的意义。

OBJECTIVE： To summarize the present state in the islets culture during islet transplantation to prepare theoretical evidences and technological support of more quality islet for transplantation by islet culture. DATA SOURCES： With the key words of ＂islet, culture＂, we searched the Medline database and Pubmed database for articles on islet culture published from January 1994 to January 2007 in English. Meanwhile, we retrieved the CNKI database for related article published from January 1994 to January 2007 with the same key words in Chinese. STUDY SELECTION： All article were selected firstly. Inclusive criteria： （1）articles on islet culture, and （2）articles published after 1994. Exclusive criterion： Meta analytic papers. DATA EXTRACTION： Among 1 384 selected articles on islet culture, 33 articles were included. All relevant information of the selected articles was summarized, including 18 articles on culture medium, culture additive, culture temperature and density, and 12 articles on new try for islet culture. DATA SYNTHESIS： Culturing islets before transplantation has several advantages over immediate transplantation such as reducing the immunogenicity of islets and gaining more time for preoperative preparation. Recently, there are many improvements on culture medium, culture additive, culture temperature, culture density, islet activity and so on. Many researchers have focus on new methods to prolong culture time and ameliorate islet function, including culturing with extracellular matrix, co-culturing with other cells, culturing under simulated microgravity conditions, packing culture with capsule and so on. CONCLUSION： Though the culture of islets is inconsistent in terms of islet survival and functional viability, new technology has greatly improved the culture condition to preserve islet viability based on the increasing knowledge about the physiology and pathophysiology of islet. It is very important and meaningful to continue the research on islet culture that is convenient in clinic.