苹果中蔗糖、葡萄糖、果糖、苹果酸的非破坏检测

Non destructive Determination of Sucrose,Glucose, Fructose and Malic Acid in Apple

探讨了用近红外光谱法非破坏检测苹果中糖、酸等四种组分的可行性，采用二阶导数来处理光学数据，分别筛选出９１４ｎｍ、９５０ｎｍ、８９７ｎｍ、９１２ｎｍ代表苹果中蔗糖，葡萄糖，果糖，苹果酸的第一特征波长。经多元线性回归分析，与高效液相色谱法相比，其复相关系数分别为０．９９７、０．９９２、０．９９２、０．９９６。对３２个预测样品的检验误差为０．０８５、０．０５７、０．１７８和０．０２１。结果表明近红外光谱法在实际应用中可满足完整苹果中糖。

The feasibility of non destructive quantitative determination of sugar and acid in intact apple by near infrared spectrometer(NIRS)was investigated.Through examination of the second derivatives of the spectra,we found that 914nm,950nm,897nm,and 912nm are the first characteristic wavelengths of sucrose,glucose,fructose and malic acid,respectively.Compared with the calibration data by high performance liquid chromatography,the results obtained at these wavelengths bear the multi correlative coefficients of 0.997,0.992,0.992,0.996 correspondingly,and the standard errors for the test samples are 0.085,0.057,0178,and 0.021,respectively.This indicates that NIRS is sufficiently precise and reliable for practical use in the quantitation of sugars and acids in intact apples.