丹参粉针剂对巨噬细胞NF-κB表达的影响

Effects of Danshen Injection on NF-κB Activation of Macrophages

目的:应用激光共聚焦显微镜技术观察丹参粉针剂对硫乙醇酸盐(TG)激活的小鼠腹腔巨噬细胞核因子-κB(nuclear factor-κB,NF-κB)表达的影响。方法:给小鼠腹腔内注射硫乙醇酸盐(TG),3天后收集小鼠腹腔巨噬细胞(macrophage),并进行纯化、培养,于培养的巨噬细胞中分别加入3个不同剂量的丹参粉针剂,孵育18h后,采用细胞免疫荧光技术对巨噬细胞进行NF-κB荧光染色,应用激光共聚焦显微镜观察巨噬细胞核内NF-κB活性的变化,并对荧光像素数进行统计分析。结果:TG刺激的小鼠腹腔巨噬细胞NF-κB表达显著升高(P<0.01);丹参粉针剂在1×10-5mg/L剂量时能显著抑制巨噬细胞NF-κB的表达(P<0.01)。结论:丹参粉针剂确能抑制TG刺激引起的小鼠腹腔巨噬细胞NF-κB的表达,推测丹参粉针剂可能通过抑制NF-κB表达而发挥抗炎作用;本研究也为丹参粉针剂的临床应用提供了进一步的理论。

Objective：To explore the inhibitory effects of Danshen injection on NF-κB activation of macrophages. Methods： With in-traperitoneal injection, Macrophages in peritoneal cavity were obtained in three days after TG injection and cultured in vitro together with Danshen injection with 3 different dosage. NF-κB in cell nuclear was observed by immunohis-tochemical method.Results ： The activation of NF-κB was significantly inhibited and nuclear translocation of NF-κB in middle dosage and positive control group was obvious. Conclusion ： Inflammatory reaction can be inhibited by Danshen injection and which provide a pharmacological basis for clinical use.