HPLC法测定灵丹草软胶囊中臭灵丹酸

Determination of pterodontic acid in Lingdancao Soft Capsula by HPLC

目的 建立HPLC法测定灵丹草软胶囊中臭灵丹酸的方法。方法 采用Agilent Zorbax XDB—C18色谱柱（250mm×4．6mm，5μm），流动相：乙腈-水（60：40），检测波长：210nm，体积流量：1．0mL／min，柱温：25℃。结果 臭灵丹酸线性范围为12．91～129．1μg／mL（r=0．9999），平均回收率为99．7％，RSD为2．0％。结论 该测定方法简便快捷、精密度高、准确性好，可以作为灵丹草软胶囊的定量测定方法，以控制其质量。

Objective To establish an HPLC method for determination of pterodontic acid in Lingdancao Soft Capsula. Methods Agilent Zorbax XDB-C18 column （250 mm×4.6 mm, 5μm） was used. Acetonitrile-water （60 ： 40） was used as mobile phase, the detection wavelength was 210 nm, flow rate was 1.0 mL/min, and column temperature was at 25 ℃. Results The linear range of pterodontic acid was 12.91-129.1μg/mL （r=0. 999 9）, the average recovery rate of pterodontic acid was 99.7 %, RSD was 2.0%. Conclusion The method is simple, accurate, and reliable, and it can be used to the quality control of this preparation effectively as a quantitative method.