重离子束注入与生物体的相互作用及遗传诱变的分子机制

Molecular Mechanism of Mutagenesis and Interaction of Incident Ions with Organism Implanted by Heavy Ions Beam

低能重离子与生物体系相互作用及其生物诱变效应的应用研究在我国率先兴起,并在应用中取得了很大的成就。介绍了重离子注入与生物有机体的相互作用及其主要的生物学效应,包括细胞染色体水平、生理生化效应,以及对DNA损伤修复、基因表达、甲基化修饰的影响。总结了离子注入诱变的分子遗传学机理的相关研究。同时分析、比较讨论了高能与低能离子、离子束与射线的生物学效应的异同。提出了离子注入今后的研究方向,特别指出了离子注入对生物基因表达影响研究的重要性。

As a new mutagenesis technique, low energy heavy ion implantation started in China for the study of interaction effect between incident ions and organism, and great achievements have been obtained in crop breeding. The article reviewed the main biological effects induced by heavy ion implantation, including physiology, biochemistry and genetics effects, on levels of cell and chromosome, gene expression, DNA methylation, DNA damage and reparation etc. It compared the differences in mutagenesis for organism by high energy and low energy ion implantation, as well as γ ray radiaiton. Future investigation topics were proposed, the emphasis of researches in future was pointed out, i. e. , the molecular mechanism and effects of gene differential expression of organism treated by ion implantation.