大豆蛋白废水生产单细胞蛋白的菌种选择及其培养条件

Selection of Yeasts and the Culture Conditions for Single Cell Protein Production from Soy Protein Wastewater by Fermentation

针对大豆蛋白生产废水的水质特性,选择了产朊假丝酵母(Candida utilis)、热带假丝酵母(Candida tropicalis)、白地霉(Geotrichum candidum link)、解脂假丝酵母解脂变种(Candida lipolytica)和扣囊拟内孢霉(Endomycopsis fiburiger)等5种工业生产酵母,作为以大豆蛋白废水生产单细胞蛋白(Single Cell Protein,SCP)的菌种,并通过摇瓶培养实验,对其培养生长条件进行了研究。结果表明,白地霉的最佳培养条件为C/N37(马铃薯-葡萄糖培养基),pH6.5,25℃,180r/min;产朊假丝酵母为C/N5(麦氏琼脂培养基),pH6,25℃,180r/min;热带假丝酵母为C/N37(马铃薯-葡萄糖培养基),pH5.5,25℃,210r/min;解脂假丝酵母为C/N37(马铃薯-葡萄糖培养基),pH5.5,25℃,180r/min;扣囊拟内孢霉为C/N37(马铃薯-葡萄糖培养基),pH6,25℃,180r/min。5种酵母菌基本都适合生长在含糖量较高,pH偏酸性,温度在25℃左右的环境当中,比较适合利用大豆蛋白废水生产SCP,为菌种的复配提供了依据,为实现利用大豆蛋白废水生产SCP奠定了基础。

Based on the characteristics of high concentration organic wastewater produced from a soy protein process, a few yeasts and the optimal culture conditions were selected to produce Single Cell Protein （SCP） by soy protein wastewater fermentation. The results of Erlenmeyer flask indicated that Candida tropicalis, Candida utilis, Candida lipolytica, Geotrichum candidum link and Endomycopsis fiburiger could be used and their optimal culture conditions were, respectively, as follows： Potato Dextrose Agar （PDA） medium, initial pH 6.5, 25℃ and 180 runs per minute （r/min） for Geotrichum candidum link; MacConkey medium, initial pH 6, 25℃ and 180 r/min for Candida utilis; PDA, initial pH 5.5, 25℃ and 210 r/min for Candida tropicalis; PDA, initial pH 5.5, 25℃, 180 r/min for Candida lipolytica; PDA, initial pH 6, 25℃ and 180 r/min for Endomycopsis fiburiger.