微米复方黄连制剂对高脂饮食喂养家兔核因子-κB及炎症因子表达的影响

Effects of micrometer compound rhizoma coptidis on nuclear factor-κB and inflammatory factors in rabbit fed with high lipid diet

目的:研究微米复方黄连制剂对高脂饮食喂养家兔炎症因子表达的影响及其可能的机制。方法:ELISA法检测血清C反应蛋白(CRP)、白介素-1(IL-1)及肿瘤坏死因子-α(TNF-α);RT-PCR法检测胸主动脉核因子-κB(NF-κB)mRNA的表达,电泳流动漂移法(EMSA)测定NF-κB的活性。结果:高脂饮食喂养16周后可明显增加血清CRP,IL-1及TNF-α的水平,并明显增加胸主动脉NF-κB mRNA的表达,同时升高NF-κB与DNA的结合活性;微米复方黄连制剂可明显逆转高脂饮食对上述指标的影响。结论:微米复方黄连制剂可明显抑制高脂饮食喂养家兔的炎症因子的表达,其机制可能是抑制NF-κB的表达及其活性所致。

To investigate the effects of the micrometer compound rhizoma coptidis on inflammatory factors and its possible mechanism in rabbit fed with high-lipid food. Method： The levels of CRP, IL-1 and TNF-a were all determinated by ELISA method. The mRNA and activity of NF-KB were determinated by RT-PCR and EMSA, respectively. Result： The level of CRP, IL-1 and TNF-a were significantly increased by feeding for 16 weeks with high-lipid diet in rabbit. It was significantly increased that the mRNA and the binging activity with DNA of NF-KB in thorax aorta of rabbits fed by high-lipid diet, too. The micrometer compound rhizoma coptidis can reverse the effects of high-lipid diet on CRP, IL-1, TNF-a and NF-kB. Conclusion： The micrometer compound rhizoma coptidis can inhibit the expression of inflammatory factor possibly through inhibitting the expression and activity of NF-kB.