荷叶生物碱柱分离纯化技术研究

Technology Study on Column Separation and Purification for Alkaloid in Lotus Leaves

以荷叶干叶为原料,经30%乙醇提取后,通过对聚酰胺、D101、HP-20三种大孔树脂对荷叶生物碱静态吸附与解吸的比较,在筛选出一种具有较高吸附率与解吸量树脂的基础上,比较研究了不同洗脱流速下不同乙醇浓度梯度洗脱对生物碱洗脱量、纯度和得率的影响以及最优流速下不同乙醇浓度等梯度洗脱对生物碱洗脱量、纯度和得率的影响。结果表明,树脂D101是三种树脂中分离纯化荷叶生物碱的最佳树脂,利用树脂D101分离纯化荷叶生物碱时,以流速为1.50BV/h依次经3BV的20%、40%、60%和80%乙醇进行梯度洗脱的技术参数较好,其生物碱总量、总得率和总纯度最高,分别达到10.8226mg、62.9%和4.86%,当单独收集80%乙醇浓度以上的洗脱液时,荷叶生物碱的纯度可达32.56%,可以制备较高纯度的荷叶生物碱。

Using the lotus leaves as raw material,after extracting alkaloids from lotus leaves by 30% ethanol, the effects of static adsorption and desorption of macroporous resin -PA（13-40）, D101 and HP-20 for lotus alkloids were compared. Then on the basis of screening the best macroporous resin with higher adsorbing rate and with desorbing amount, the effects of the desorbing quantity,purity and yield rate of alkloids at different eluting velocities by the desorption gradient of different eluting density of ethanol with volume 3 BV were studied, and the effects of desorbing quantity, purity and yield rate of alkloids by the same gradient of different eluting density under the best eluting velocity were also studied. The results showed that D101 is the best among the three kinds of resin. To separate and purify lotus alkaloids by D101 at 1.5 BV/h with 3 BV desorbing volume of ethanol concentration 20%, 40%, 60%, 80% ethanol gradient desorption is proved to be the best, with the total quantity of eluted alkaloid, yield rate, the total purity reaching 10.8226 rag, 62.9%, 4.86% in sequence. When only collecting the desorbing liquid with concentration above 80% ethanol, the purity of lotus alkaloids can reach 32.56%, which is valuable to process higher purity lotus alkaloids.