复合诱变选育γ-癸内酯生产菌株

Breeding of Strain for Producing γ-Decanolactone by Composite Mutation Method

以紫外诱变和化学诱变相结合的复合诱变方法,对γ-癸内酯产生菌株Yarrowia lipolytica酵母进行诱变。经过一次紫外诱变后,γ-癸内酯产量为过去的2.5倍,但多次诱变后其产量并没有进一步提高,且稳定性不好。其后采用硫酸二乙酯进行化学诱变稳定其产量。最终γ-癸内酯产量为出发菌株的2.3倍。

A strain for producing γ-decanolactone was obtained by composite mutation （ultraviolet mutation and chemical mutation）. After the first ultraviolet mutation, the yield of γ-decanolactone was as 2.5 times as the original strain. But the yield was not stability and didn＇t have further increase after more mutations. At last diethyl sulfate stabilizexi the yield, and it was increased by 2.3 times.