酶法提取蛋壳膜中的角蛋白

Enzyme Extraction of Keratin from Eggshell Membrane

以鸡蛋蛋壳膜为原料,酶法提取蛋壳膜中角蛋白的研究,对酶的用量、提取时间、提取温度、提取的最佳pH值、反应的固液比进行了探讨,在最佳工艺条件下:酶用量为蛋膜量的3%(W/W),固液比1:15,pH9～10,提取温度55～65℃,提取时间60min,角蛋白产率可达到26%。

In this paper, the principle of experiment and operation method of extracting keratin from eggshell membrane with alkaline protease was introduced. From the results, the optimum conditions were obtained, including the quantity of alkaline protease 2%（W/W）, solid-liquid ratios 1：15, pH9- 10, temperature 55-65 ℃, and reaction time 60 min etc. The keratin yield reaches 26%.