增生性玻璃体视网膜病变增生膜中基质金属蛋白酶及其抑制剂的表达

Expression of matrix metalloproteinases and its inhibitors in proliferative vitreoretinal membranes

目的研究增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)增生膜中基质金属蛋白酶(matrix metalloproteinases,MMP)及其抑制剂(tissue inhibitors of ma-trix metalloproteinases,TIMP)的表达。方法采用免疫组织化学技术的SP法,检测PVR增生膜和正常尸眼神经视网膜标本中MMP-2、MMP-9和TIMP-1的表达,光镜观察染色结果。结果41例PVR增生膜标本HE染色切片光镜下可见视网膜色素上皮(retinal pig-ment epithelial,RPE)细胞、神经胶质细胞、成纤维细胞、巨噬细胞等细胞成分,它们被大量细胞外基质所包绕。视网膜前膜中以RPE细胞为主要增生细胞,视网膜下膜中以神经胶质细胞为主要增生细胞。免疫组织化学染色结果显示:(1)25例PVR增生膜标本表达MMP-2,11例PVR增生膜标本表达MMP-9,14例PVR增生膜标本表达TIMP-1;(2)22例视网膜前膜标本表达MMP-2,3例视网膜下膜标本表达MMP-2,差异有显著性(P<0·05);(3)正常尸眼神经视网膜标本中未检测到MMP-2、MMP-9及TIMP-1的表达。结论细胞外基质与PVR增生膜的形成关系密切,MMP-2、MMP-9及TIMP-1在PVR形成过程中发挥重要作用,通过合理地调控MMP和TIMP的平衡,为预防和治疗PVR提供可能性理论依据。

Objective To examine the expression of matrix metalloproteinases （MMPs） and tissue inhibitors of matrix metalloproteinases （TIMPs） in the membranes of proliferative vitreoretinopathy（PVR）. Methods The expression of MMP-2,MMP-9 and TIMP-1 in PVR membranes and normal cadaverie nervotm retina were examined by SP method of immunohistochemical technology. The results were observed by optical microscope. Results The retinal pigment epithelial （RPE） cells,nettroglial cells,fibroblasts,maerophage were seen and surrounded by extracellular matrix in 41 cases of PVR membranes stained by HE. RPE cells were main proliferative cells in epiretinal membranes, while nettroglial cells in subretinal membranes. The results of immunohistochemical methods showed that： （ 1 ） MMP-2 was expressed in 25 cases of PVR membranes,while MMP-9 in 11 cases,TIMP-1 in 14 cases;（2）MMP-2 was expressed in 22 cases of epiretinal membranes and 3 cases of subretinal membranes,there was significant difference（ P 〈 0.05 ） ; （ 3 ） The expression of MMP-2, MMP-9 and TIMP-1 wash＇t detected in normal cadaverie retinas. Conclusion Extracellutar matrix has a close relationship with formation of PVR membranes, and MMP-2 ,MMP-9 and TIMP-1 play an important roles in formation of PVR membranes. By controlling the balance of MMPs and TIMPs reasonably may provide a possible theory in preventing and curing PVR.