摘要
目的采用等度洗脱的高效液相色谱法测定多维元素片（29）中维生素K1的含量。方法迪马C18（250mm×4.6mm,5μm）,柱温25℃,流动相无水乙醇-水（92∶8）,流速1.0mL/min,检测波长254nm。结果维生素K1在0.1812~0.7248μg范围内线性关系良好（r=0.9999）,平均回收率为99.89%,RSD为0.67%。结论本法降低了仪器要求,简化操作,准确可靠,可用于维生素K1的质量控制。
Objective To determine the content of vitamin KI in multivitamin and minerals tablet （29） by RPHPLC. Methods RP-HPLC was used with C18 column, and the mobile phase consisted of dehydrated alcohol-water （92 ：8） with the detection wavelength at 254 nm. The flow rate was at 1.0 mL/min and the column temperature was 25 . Results The standard curve was liner over the range of 0. 181 2- 0. 724 8 μg （r = 0. 999 9）, and the average recovery was 99.89% with RSD = 0.6796. Conclusion The method degrades the requirement of equipment, and it is simple, accurate and reliable. It can be used for the quality control of vitamin K1.
出处
《实用药物与临床》
CAS
2007年第3期189-190,共2页
Practical Pharmacy and Clinical Remedies
