运用RT-PCR一步法和Nested-CR法快速检测FMDV的研究

Studies on the Rapid Detection of Foot and Mouth Disease Virus Using One Step RT PCR and Nested PCR

将Ｏ、Ａ型ＦＭＤＶＲＮＡ分离纯化，用ＲＴ－ＰＣＲ法扩增其聚合酶编码基因，可检测到Ａ型１０－６倍稀释、Ｏ型１０－４倍稀释乳鼠肌肉毒。Ｎｅｓｔｅｄ－ＰＣＲ扩增该基因内部片段，进一步说明该方法准确可靠。ＲＴ－ＰＣＲ一步完成使整个检测时间不超过１２小时。

An RT PCR was developed to amplify the RNAs of Types A and O FMDV.The sensitivity reached 10 -6 for Type A and 10 -4 for Type O.Amplification of the inner fragments of the gene confirmed the reliabilty for the detection of FMDV.The completion of the whole RT PCR was no more than 12 hours .It is suggested that RT PCR be applied for the import quarantine diagnosis and detection of FMD.