昆明系和Balb/C系小鼠用于显性致死试验的比较研究

COMPARATIVE STUDY OF KUNMING AND BALB/C STRAIN MICE FOR DOMINANT LETHAL TEST

本研究选用昆明系和Ｂａｌｂ／Ｃ系小鼠同时进行了显性致死试验的对比研究。试验一选用封闭群昆明系９～１０周龄的雄性小鼠７８只，随机分成６组，其中５个剂量组分别灌服１６０、５３、１６ｍｇ／ｋｇ体重的复方硝基酚钠和１５、３０ｍｇ／ｋｇ体重的环磷酰胺，另一组为空白对照组。受试物组每天给药一次，连续５ｄ后，按１∶２的雄雌比例与正常的９～１０周龄封闭群昆明系雌鼠进行交配，每周交配一批，连续交配８批。试验二除选用近交系Ｂａｌｂ／Ｃ雄鼠取代昆明系雄鼠外，其它条件与方法同试验一。试验结果表明，各批雌鼠各组之间的受孕率、着床数均无明显差异；两个试验中１５、３０ｍｇ／ｋｇ环磷酰胺组的第１、第２和／或第３批的活胎数明显低于其它各组，死胎数、有一个以上死胎的孕鼠率及显性致死率明显高于其它各组，显性致死试验结果为阳性；而不同剂量的复方硝基酚钠组的各项指标与空白对照组比较未见明显差异。本研究中雌雄试验动物选用同一个品系的昆明系小鼠进行显性致死试验的结果与选用Ｂａｌｂ／Ｃ系雄鼠与昆明系雌鼠进行杂交的结果比较无明显差异。

This report deals with the dominant lethal test in two trials of Kunming and Balb/C strain mice.In the 1st trial,78 male mice of closed Kunming strain were selected and divided into 6 groups (13 per group):3 groups(160,53,16mg/kg b w )of composed sodium nitrophenolates(CO NP Na),2 groups(30,15mg/kg b w )of cyclophosphamide and 1 control group After administering tested medicine for 5 days by oral,the male mice of each group mated with the Kunming female mice(1∶2),the female mice were exchanged each week,the male mice mated for 8 batches The method of the 2nd trial was the same as the 1st trial except substituting Balb/C strain male mice for Kunming strain male mice The results show that there were not significant differences on fertility and No of implants of each batch female mice In the 15 and 30mg/kg cyclophosphamide groups of two trials,No of living fetuses was significantly decreased and No of dead fetuses,ratio of pregnant mice which had more than one dead fetuses,rate of dominant lethal were significantly increased in the 1st,2nd and/or 3rd batches female mice The dominant lethality of cyclophosphamide was positive Comparing with the control group,the results of each group of CO NP Na in two trails were not significantly different In conclusion,the different strain of mice does not influence the results of dominant lethal test