摘要
作者研究了孔雀绿-胆碱磷钼杂多酸-PVA体系,提出了一种相对简便、灵敏测定植物材料中痕量总胆碱的方法.方法的线性范围为0.5~200nmol/L,回收率大于85%.
A simple and sensitive spectrophotometric method for assay of choline which had been developed for analysis of total choline in plant materials was reported here. The conditions of complex formation of malachite green, choline phospho molybdoheteropoly acid and polyvinyl alcohol (PVA) were studied.The calibration graph was linear in the range of 0.5~200 nmol/L for choline. The recovery rate was greater than 85%.
基金
浙江省分析测试基金
关键词
胆碱
孔雀绿
分光光度法
植物材料
choline
malachite green
spectrophotometry
plant materials