通络救脑口服液对AD大鼠学习记忆能力及AChE表达的影响

Effect of Tongluo Jiunao Oral Solution on Learning and Memory and the Expression of AChE in Hippocampus of AD Rats

目的:观察通络救脑口服液对β-淀粉样蛋白1-40诱导的AD大鼠学习记忆能力及乙酰胆碱酯酶(AChE)表达的影响。方法:140只SD大鼠随机分为空白对照组(正常组)、假手术对照组、阴性对照组(模型组)、阳性对照组(盐酸多奈哌齐组)、实验组(48、24、12mg.kg-1.d-1)共7组。采用大脑海马立体定向注射凝聚态β-淀粉样蛋白1-40诱导老年性痴呆(AD)动物模型。药物干预4周后,采用Morris水迷宫测试大鼠寻找平台所需时间和通过原平台次数。采用免疫组织化学染色和计算机图像分析技术测定海马区AChE蛋白阳性目标积分光密度。结果:Morris水迷宫实验:模型组大鼠在定位航行试验中隐匿平台平均逃避潜伏期较正常组显著延长,空间探索试验中跨越原平台位置次数明显减少(P<0.01),通络救脑口服液各剂量组平均逃避潜伏期较模型组显著缩短(P<0.05),跨越原平台位置次数明显增加(P<0.01)。免疫组化实验:模型组和正常组大鼠海马区AChE蛋白阳性目标积分光密度比较无显著性差异(P>0.05),但与通络救脑各剂量组比较差异显著(P<0.01)。结论:通络救脑口服液对Alzheimer病模型大鼠的学习记忆功能减退具有改善作用,可能与抑制海马神经元AChE表达,进而减少乙酰胆碱(ACh)的分解有关。

Objective： To observe the effect of Tongluo Jiunao oral solution on learning and memory and the expression of AChE in hippocampus of AD rats induced by β-amyloid1 -40. Methods： 140 SD rats were divided into blank control group （normal group）, pseudo- operation control group, negtive control group （model group）, positive control group and experiment group（48,24,12mg· kg^-1·d^-1 ）at random. The model of AD were established by injecting β-amyloid1-40 into bilateral hippocampus. After 4 weeks, Morris Water Maze was used to determine the ability of learning and memory. Immunohistochemical method and image analysis techniques were used to determine the expression of AChE in Hippocampus. Results： Compared with normal group, in place navigation test, the mean escape latencies of the model group increased obviously, and in spatial probe test, the frequency of passing through the platform of the model group decreased obviously （ P 〈 0.01 ）. Compared with model group , the mean escape latencies of Tongluo Jiunao oral solution group（48,24,12 mg· kg^-1·d^-1 ） decreased obviously （ P 〈 0.05 ）, and in spatial probe test, the frequency of passing through the platform of Tongluo Jiunao oral solution group （48,24,12mg· kg^-1·d^-1） increased obviously（ P 〈 0.01 ）. The expression of AChE had no difference between model group（ P 〈 0.01 ） and normal group（ P 〈 0.05）. But compared with Tongluo Jiunao oral solution group, it had obviously significant difference. Conclusion： Tongluo Jiunao oral solution can reduce the disassociation of ACh by restraining the expression of ACHE, and then improve the ability of learning and memory of AD rats.