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PCR扩增ZFY、ZFX序列鉴定奶牛性别的灵敏度研究 被引量:10

Sensibility of Sex Identification with Amplification of ZFY and ZFX Gene Sequences in Bovine by PCR
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摘要 从克隆、测序所得奶牛ZFY、ZFX序列中设计1对高特异性引物(或探针),即分别特异于牛ZFY、ZFX的ZF3、ZF4,与克隆时使用的引物ZF2搭配成ZF2、ZF3和ZF2、ZF4。利用这2对引物和ZF1、ZF2对奶牛基因组DNA进行NestPCR来判断性别,结果达到用超微量(8pg)DNA,即可检出ZFY、ZFX序列而判定雌雄的灵敏度;用非同位素标记试剂盒(DIG-System)标记ZF3、ZF4作为探针,对ZF1、ZF2扩增产物点杂交来判断性别。 The sequences of ZFY and ZFX gene fragments obtained from cloning and sequencing in bovine were analyzed. It was found that there were 88.7% homologous region within the two fragments. The remains appeared to be different. According to these different sequences, the primers or probe (ASO) ZF 3 specific for ZFY and the ZF 4 specific for ZFX were designed. One set of ZFY specific primers formed by ZF 2 and ZF 3 and the another set of ZFX specific primers formed by ZF 2 and ZF 4 were used to identify the sex of bovine by nest PCR. The results showed that a 8 pg DNA level of the sensibility for sex identification achieved in bovine by the nest PCR.
出处 《中国兽医学报》 CAS CSCD 北大核心 1997年第3期234-237,共4页 Chinese Journal of Veterinary Science
基金 复旦大学国家基因工程重点实验室项目 国家民委"八五"重点项目
关键词 性别鉴定 PCR ZFY ZFX bovine sex identification PCR ZFY ZFX
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