磷对奶牛红细胞膜脂质的影响

Effect of Phosphorus on Erythrocyte Membrane Lipids in Cows

对１５例临床上有明显低磷血症的奶牛血清磷、红细胞膜脂质成分进行了测定。结果表明，低磷血症奶牛血清磷、红细胞膜磷脂明显降低；红细胞膜胆固醇及膜胆固醇／磷脂摩尔比值（ｎｃｈ／ｎｐｌ）明显升高，与健康对照组牛相比差异极显著（Ｐ＜０．０１）；直线相关分析表明，血清磷与红细胞膜磷脂之间呈极显著正相关（ｒ＝０．９１７，ｙ＝１０．８５２ｘ＋３．１９６，Ｐ＜０．０１）；与红细胞膜胆固醇以及ｎｃｈ／ｎｐｌ之间分别呈显著负相关（ｒ＝－０．９４０，ｙ＝２．８５０ｘ－１．０７２，Ｐ＜０．０１；ｒ＝－０．９２０，ｙ＝１．９６８ｘ－１．４０１，Ｐ＜０．０１）。此外，红细胞膜微粘度与膜磷脂之间呈显著负相关（ｒ＝－０．９５４，ｙ＝１９．１２２ｘ－４．３８４，Ｐ＜０．０１），而与膜胆固醇以及ｎｃｈ／ｎｐｌ之间呈显著正相关（ｒ＝０．９８８，ｙ＝０．９２７ｘ＋０．９６４，Ｐ＜０．０１；ｒ＝０．９７８，ｙ＝－０．２９３ｘ＋１．１１３，Ｐ＜０．０１）。结果表明，磷是红细胞膜脂质成分改变的先决因素，而后者又是红细胞膜流动性及膜结构、功能发生变化并导致膜损伤的一个重要因素。

Serum inorganic phosphorus and membrane lipids of erythrocyte of 15 cows suffered from obviously clinical hypophosphatemia were measured. The results showed that serum phosphorus, erythrocyte membrane phospholipids had a significant decrease (P<0.01) while the content of erythrocyte membrane cholesterol and its molar ratio of cholesterol/phospholipids were significantly increased (P<0.01) as compared with those of the health control group.By stepwise regression analysis, the serum phosphorus was shown to be significantly positively related to membrane phospholipids of erythrocyte (r=0.917, y=10.852x+3.196, P<0.01), but significantly inversely to cholesterol and cholesterol/phospholipids ratio (r=-0.940, y=2.850x-1.072, P<0.01; r=-0.920, y=1.968x-1.401, P<0.01), and the viscosity significantly inversely related to membrane phospholipids of erythrocyte (r=-0.954, y=19.122x-4.384, P<0.01), but significantly positively to cholesterol and cholesterol/phospholipids ratio (r=0.988, y=0.927x+0.964, P<0.01; r=0.978, y=-0.293x+1.113, P<0.01). This indicated that phosphorus deficiency might be the most important predisposing factor for the changes of membrane lipid of erythrocyte which would be an important reason for the changes of erythrocyte membrane fluidity and other structure, function or even hemolysis.