固相RIA法测定血浆孕酮监测黄牛产后卵巢机能

Determination of Plasma Progesterons by Solid Phase RIA for Monitoring Postpartum Ovarian Activity of Luxi Cattle

应用１２５Ｉ固相放射免疫分析法，对２２头鲁西母黄牛产后期血浆孕酮（Ｐ４）的变化作了测定，以监测黄牛产后卵巢机能。结果表明，产后（１３．６±４．４）ｄ以前，母牛血浆Ｐ４浓度仍处于基础水平〔（０．３４±０．０４）～（０．５５±０．０６）μｇ／Ｌ〕，随后，Ｐ４开始升高，出现卵巢活动。代表卵巢活动的Ｐ４曲线可分为４种类型：Ⅰ型，产后（１０．６±３．９）ｄ后，曲线呈现３个正常的黄体周期；Ⅱ型，产后（１３．６±４．４）ｄ后，首先出现１个８．５ｄ左右的短黄体周期，接着出现２个正常的黄体周期；Ⅲ型，于产后（１２．５±３．９）ｄ后，先出现１个正常的黄体周期，继之出现１个短黄体周期和１个正常黄体期；Ⅳ型，产后（１２．５±２．５）ｄ后，出现不规则的黄体周期中，最低Ｐ４水平为（０．４５±０．１５）～（０．６０±０．３８）μｇ／Ｌ，最高浓度为（２．６５±１．９５）～（４．１７±２．３５）μｇ／Ｌ。试验表明，每隔４ｄ测定１次血浆Ｐ４浓度。

The blood samples were collected from 22 head of Luxi cattle, aged 2.5￣8, from calving day (day 0) to 80 days postpartum at 4 day intervals. Progesterone (P 4) levels in plasma were determined by solid phase RIA. The results are summarized as follows: P 4 levels in plasma of the cows remained at (0.34±0.04) (0.55±0.06) μg/L during (13.6±4.4) days postpartum, then began to rise and the ovarian activity appeared. The plasma P 4 profiles of the cows can be classified into 4 types, characterized by (Ⅰ) 3 normal cycles; (Ⅱ) a short cycle followed by 2 normal cycles; (Ⅲ) a normal cycle followed by a short cycle and a normal cycle; and (Ⅳ) some irregular cycles respectively. The lowest and the highest P 4 levels were (0.45±0.15) (0.60±0.38) μg/L and (2.65±1.95) (4.127±2.35) μg/L respectively in luteal cycles. The determination of plasma P 4 concentrations at 4 day intervals was shown to be able to identify precisely the oestrus cycles of cows.