矽肺及肺结核患者外周血T淋巴细胞亚群的检测及分析

Detection and Analysis of T lymphocyte Subpopulation in Peripheral Blood of Patients with Silicosis and Tuberculosis

[目的]探讨T淋巴细胞亚群在矽肺和肺结核发生中的变化,为指导职业人群矽肺病和肺结核的预警、治疗及应用免疫增强剂等提供依据。[方法]采用流式细胞术检测矽肺患者45例、肺结核25例及对照组60例的外周血T淋巴细胞亚群。[结果]矽肺和肺结核组CD_3^+、CD_4^+T细胞、CD_4^+/CD_8^+比值均低于对照组(P＜0.05),肺结核组CD_8 T细胞高于对照组(P＜0.05)。[结论]矽肺和肺结核患者免疫功能低下,T淋巴细胞亚群的检测,对监控患者的免疫状态、指导临床治疗、观察疗效及预后具有重要意义。

[Objective] To study the alteration of T lymphocyte subpopulation in silicosis and tuberculosis and guide the disease warning, treatment and application of immunity intensifier for occupation population. [Methods] With casecontrol study （45 silicosis, 25 tuberculosis patients and 35 healthy people）, T lymphocyte subpopulation in peripheral blood was detected by using flow cytometry method. [Results] CD3^＋ and CD4^＋ T cell, CD4^-/CD8^- ratio in silicosis and tuberculosis group were lower than those of healthy people （P〈0.05） and CD8^＋ T cell of tuberculosis group was higher than that of healthy people （P〈0.05）. [Conclusions] The immunity function of silicosis and tuberculosis patients is at lower level. And the detection of T lymphocyte subpopulation plays an important role in monitoring patients＇ immunity status, guiding clinical treatment, evaluating of therapeutic effect and prognosis.