CD146和MMP-2在人肝细胞癌组织中的表达及临床意义

Expressions and clinical significance of CD146 and MMP-2 in hepatocellular carcinoma

目的:研究人肝细胞癌(hepatocel-lular carcinoma,HCC)组织中CD146及基质金属蛋白酶-2(matrix metalloproteinase2,MMP-2)的表达,并探讨其与血管生成的关系。方法:采用免疫组化法检测60例肝癌组织及癌旁组织中CD146和MMP-2的表达情况,用CD34标记免疫组化法检测微血管密度(microvessel desity,MVD)。结果:CD146和MMP-2在癌组织中的阳性率分别为66.67%和60.00%,而在癌旁组织中的阳性率分别为30.00%和26.67%,癌组织与癌旁组织比较差异有统计学意义,P<0.01。癌组织的MVD为54.92±8.55,癌旁组织的MVD为21.36±6.63,两者差异有统计学意义,P<0.01。CD146在人肝癌组织中的表达与临床分期、门静脉癌栓及肝外转移相关,而与术后复发、肿瘤个数、肿瘤直径、血清AFP水平及肿瘤分化程度无明显关系。MMP-2在人肝癌组织中的表达与临床分期、门静脉癌栓、肝外转移及术后复发相关,而与肿瘤大小、肿瘤个数、血清AFP水平及肿瘤分化程度无明显关系。在癌组织中MVD与CD146、MMP-2的表达呈正相关,CD146与MMP-2亦呈正相关。结论:肝癌组织中CD146及MMP-2高表达,与肿瘤血管形成有关。

OBJECTIVE： To study the expressions and significance of CD146 and matrix metalloproteinase 2 （MMP-2） in human HCC （hepatocellular carcinoma） tissues, and to explore their correlation with angiogenesis of HCC. METHODS： Immunohistochemisty was employed to determine the expressions of CD146, MMP-2 and microvessel desity （MVD） in 60 HCC tissues and their adjacent liver tissues. RESULTS.. The positive rates of CD146 and MMP-2 in the HCC tissues were higher than those in the adjacent liver tissues （66.67% and 60.00% vs. 30.00% and 26.67%, P〈0.01）. MVD in the HCC tissues was significantly higher than that in the adjacent liver tissues （54.92±8.55 vs. 21.36±6.63,P〈0.01）. The expression of CD146 in the HCC tissues was significantly correlated with the clinical stage, the portal vein tumor thrombus and the presence of extrahepatic metastasis, hut not correlated with the recurrence of tumor, the number of tumor, the diameter of tumor, the level of serum alpha-fetoprotein （AFP）, and the differentiation of tumor. The expression of MMP-2 in the HCC tissues was significantly correlated with the clinical stage, the portal vein tumor thromhus, the presence of extrahepatic metastasis and the recurrence of tumor, hut not correlated with the diameter of tumor, the number of tumor, the level of serum alpha-fetoprotein （AFP）, and the differentiation of tumor. MVD was positively correlated with the expressions of CD146 and MMP-2, and the expression of CD146 was positively correlated with that of MMP-2. CONCLUSION： There are high expressions of CD146 and MMP-2 in HCC tissues, which suggests that CD146 and MMP-2 may play important roles in angiogenesis of HCC.