非小细胞肺癌组织中SKP2蛋白的表达及其对预后的影响

Expression of SKP2 protein in non-small cell lung cancer and its affection to the prognosis

目的:探讨SKP2蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其对患者预后的影响。方法:利用组织芯片和免疫组化技术检测SKP2蛋白在89例NSCLC、5例肺良性肿瘤和5例正常支气管和肺组织中的表达。结果:NSCLC组织中SKP2蛋白表达阳性率为(23·52±13·57)%,明显高于肺良性肿瘤、正常支气管及肺组织(2·91±1·27)%,两者差异有统计学意义,t′=31·373,P=0·000。SKP2蛋白在NSCLC组织中的表达水平与细胞分化程度密切相关,χ2=14·402,P1=0·000;与年龄、性别、吸烟史、肿瘤位置、大小、病理类型、淋巴结转移和TNM分期等差异无统计学意义,P1值均>0·05。SKP2蛋白表达阳性的NSCLC患者5年生存率较阴性患者降低,χ2=4·119/4·636,P1=0·042/0·031;r=-0·186,P2=0·000。结论:SKP2蛋白表达在NSCLC的发生发展中起促进作用,并对患者的预后产生不良影响。

OBJECTIVE： To research the expressive characteristics of SKP2 protein in non-small cell lung cancer and its affection to NSCLC patient＇s prognosis. METHODS： The expression of SKP2 protein was detected in 89 NSCLC, 5 benign lung neoplasmas, 5 normal bronchus and lung tissues by the tissue chip and immuno-histochemistry technology. RESULTS： The positive rate of SKP2 protein stain was （23. 52±13. 57）% in NSCLC tissues, which was significantly higher than that in benign lung neoplasms, normal brochus and lung tissues （2. 91±1.27）%, t＇=31. 373,P=0. 000. The expressive level of SKP2 protein in NSCLC tissues was closely related to the cell differentiation （X^2 = 14. 402, P1 =0.000）, but not to age, sex, smoking history, neoplasmtic pathological type, site, size, lymph node metastasis, TNM stage etc. , each P1 〉0. 05. The survival analysis displayed the 5-year survival rate of NSCLC patients was lower in positive expression of SKP2 protein group than that in negative expression of SKP2 protein one, X^2 =4. 119/4. 636, P1 =0. 042/0. 031;r= -0. 186, P2 =0. 000. CONCLUSIONS： Positive expression of SKP2 protein may play an enforcement role in occurrence and development of NSCLC. Moreover, it may bring bad affection to NSCLC patient＇s prognosis.