肿瘤相关基因1A6/DRIM与胃癌生物学特性关系的研究

Relationship between the expreaaion of a tumor related gene-1A6/DRIM and the tumor biological characteristics of gastric carcinomas

目的:探讨肿瘤相关基因1A6/DRIM表达与胃癌生物学特性的关系,及其在胃癌临床病理诊断中的应用价值。方法:应用自行制备鼠抗人1A6/DRIM单克隆抗体,采用SP免疫组织化学方法检测100例不同类型胃癌组织及对应远端正常组织中1A6/DRIM的表达。结果:在100例胃癌组织中,呈1A6/DRIM基因阳性表达者66例;在癌旁正常组织中其阳性表达者12例,两者比较具有显著统计学差异(χ2=63.291,P=0.000)。进一步结合临床病理资料统计分析发现,1A6/DRIM在胃癌组织中表达与肿瘤的分化程度、胃壁浸润、淋巴结转移有关(χ2分别为5.414、7.913、7.778,P值分别为0.02、0.005、0.005),均有显著差异;而其表达与肿瘤远处转移、病人年龄、性别无关(χ2分别为3.488、0.163、0.221,P值分别为0.062、0.687、0.639)。结论:1A6/DRIM作为新的肿瘤相关基因,在胃癌诊断中作用明确,并且在判断肿瘤分化程度、浸润、淋巴结转移等方面具有较好的辅助诊断价值,可逐步应用于临床。

Objective To investigate the relationship between the expression of 1A6/DRIM, a tumor-related gene and the tumor biological characteristics in human gastric carcinomas, and to elucidate its clinical and pathological diagnostic value. Methods The gene 1A6/DRIM was a self-made by means of a mouse anti human 1A6/DRIM monoclonal antibody, and immunohistochemical S-P method was used in studying 100 cases of gastric carcinomas and 100 specimens of correlated normal gastric tissues from surgical resections. Results The positive rate of 1A6/DRIM expression in gastric carcinomas was 66% while the positive rate in normal gastric tissues was 12%, a significant difference existed between them （χ2=63.291 ,P=0.000）; 1A6/DRIM overexpression was related with the poorly differentiated adenocarcinomas （χ2= 5.414,P=0.02）, the depth of invasion（χ2=7.913,P=0.005）, lymph node metastasis（χ2=7.778,P=0.005）, while not related with age（P=0.687）, sex（P=-0.639） and distant metastasis （P=-0.062）. Conclusions As a new tumor-related gene, 1A6/DRIM may play an important role in the formation and development of gastric carcinomas, The expression of 1A6/DRIM could be employed as a useful marker in the clinical and pathological diagnosis of gastric carcinomas.