斯氏艾美耳球虫孢子生殖及其致病性研究

Observation on the sporulation and pathogenicity of Eimeria stiedai

目的采用单卵囊分离技术分离斯氏艾美耳球虫(Eimeria stiedai),观察其卵囊在孢子化过程中的发育形态变化。方法通过人工感染断奶幼兔,以潜伏期、死亡率、肝指数、血清中转氨酶数值、肝脏病变记分等指标研究其致病性。结果采用单卵囊感染技术成功分离了E.stiedai单卵囊,卵囊在29℃条件下最早孢子化形成时间为39 h,潜在期为13～15 d,感染率为20%。结论用继代增殖的纯株E.stiedai人工接种试验兔,在一定范围内其致病性随感染剂量的增加而增强。

Objective To isolate the pure specie of Eimeria stiedai by single oocyst separation technology and observe the sporulation process. Methods The pathogenicity of isolated specie was studied by a series of indexes such as latency period, mortality, liver index, transaminase, scores of pathological changes in artificial infected young rabbits. Resuits The shortest time of sporulation of oocyst was 39 hours under the temperature of 29℃. The latency period was 13 to 15 days and the infection rate was 20 %. Conclusion The experiment results indicated that the pathogenicity of pure specie of E. stiedai was proportional to the inoculated dose of continuous proliferative oocysts in a range in the inoculatd groups.