IFN-γ、L-Arg及L-NNA对感染旋毛虫的沙鼠血液及腓肠肌NO合成和组织病理变化的影响

Effects of IFN-γ,L-Arg and L-NNA on NO production and tissues pathology change in serum and gastrocnemius of Mongolian jirds infected Trichinella spiralis

目的探讨IFN-γ、L-Arg和L-NNA对感染旋毛虫的沙鼠血液及腓肠肌NO合成和组织病理变化的影响。方法经腹腔给予感染旋毛虫后不同时期的沙鼠不同剂量的IFN-γ、L-Arg和L-NNA,检测沙鼠血清及腓肠肌NO的含量。观察腓肠肌的病理变化。结果IFN-γ和L-Arg组沙鼠血清及腓肠肌NO含量随药物浓度的增加而升高;L-NNA阴性对照组血清NO含量和感染25 d的L-NNA组腓肠肌NO含量升高,L-NNA实验组血清NO含量及阴性对照组、旋毛虫感染7 d组4、0 d组腓肠肌NO含量降低。实验组腓肠肌内囊包数较阳性对照组略有减少。结论IFN-γ和L-Arg能促进感染旋毛虫的沙鼠血液及腓肠肌NO合成,L-NNA则具有增强或抑制NO合成的双重作用。

Objective To study the effects of Interferon-γ （IFN-γ）, L-arginine（L-Arg） and NC-Nitric-L-arginine（L- NNA） on NO production and tissues pathology change in blood and gastrocnemius of Mongolian jirds infected Trichinella spiralis. Methods Different doses of IFN-γ, L-Arg and L-NNA were injected into the different grouped jirds by abdominal cavity in different time after infection. The levels of NO production of serum and gastrocnemius of jirds were de- tected. Gastrocnemius pathology of jirds was determined. Results In the blood and gastrocnemius the NO contents of IFN-γ group and L-Arg group were raised with raising concentration of IFN-γ and L-Arg. It was raised that the NO contents of serum in L-NNA uninfected control group, and of gastrocnemius in L-NNA infected 25 d group. It was depressed that the NO contents of serum in L-NNA infected group, and of L-NNA uninfected control group, infected T. spiralis 7 d and 40 d groups. The amounte of larvae in experimental groups was slightly reduced compared with positive control group. Conclusion IFN-γ and L-Arg can promote synthesis of NO in blood and gastrocnemius of jirds infected T. spiralis. L-NNA shows dual effects of promotion or inhibition in NO synthesis of blood and gastrocnemius.