β-catenin在胎牛蹄边缘毛囊形态发生中表达的时空变化

Temporal and Spatial Expressions of β-Catenin on The Hair Follicle Morphogenesis of The Hoof Periphery in Bovine Embryos

以胎牛为实验动物,应用免疫组化方法定性检测β-catenin在胎牛蹄边缘表皮和毛囊形态发生中表达的时空变化,探讨胎牛蹄边缘毛囊形态发生中β-catenin表达的时空变化规律.结果显示:β-catenin在胎牛蹄边缘毛囊形态发生早期(68～93天)表达于表皮、基底层、基板和毛芽中,其中基底层、基板、毛芽中呈强阳性,表皮中呈中阳性;在毛囊形态发生中期(94～184天)表达于基底层、表皮和毛钉中,其中基底层、表皮、隆突区、内外根鞘和漏斗部呈中阳性;在毛囊形态发生晚期(184～225天)只在表皮及基底层有表达,其中基底层呈弱阳性,表皮呈强阳性.β-catenin表达的时空变化规律提示,在胎牛蹄边缘毛囊的形态发生过程中β-catenin具有重要作用.

Temporal and spatial expressions of β-catenin investigated in the hair follicle and epidermis of the hoof periphery in bovine embryonic development. IHC （immunohistochemical method） was applied to qualitatively detect the temporal and spatial expressions of β-catenin. β-catenin was detected in suprabasal, epidermal basal layer, placode, hair bud in early phase（E68-93）,and expressed strongly in epidermal basal layer, placode, and hair bud, in suprabasal expressed less strongly; in metaphase（E94 - 184）, β-catenin was detected in epidermis, hair peg, and in suprabasal, epidermal basal layer, hair follicle bulge, inner root sheath, outer root sheath, follicular infundibulum expressed less strongly; in late phase（E184-225）, β-catenin expressed weakly in epidermal basal layers, while expressed strongly in epidermal keratinocytes. The result suggested that β-catenin plays an important role in hair follicle morphogenesis in the periphery of bovine hoof in bovine embryos.