内毒素致兔肝线粒体膜磷脂含量改变及阳离子A的拮抗效应

Effect of endotoxin on phospholipids of hepatic mitochondria membrane and cation A antagonism in rabbits

目的:采用内毒素血症兔实验模型,研究肝线粒体膜磷脂含量的改变及阳离子A的拮抗效应。方法:将48只日本大耳白兔随机分为正常对照组(Ⅰ组)、内毒素组(Ⅱ组)和阳离子A拮抗组(Ⅲ组)。3组同时进行相应处理后,分别在第3、7h测定各组肝线粒体膜中磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)和磷脂酰肌醇(PI)的含量变化。结果:Ⅱ组中PC、PE、PS、PI含量在第3h、7h均低于Ⅰ组,差异极显著(P<0.01);而Ⅲ组中4种磷脂的含量在第3h、7h又均高于Ⅱ组(P<0.05,P<0.01)。结论:内毒素致使兔肝线粒体膜磷脂含量降低,而阳离子A一定程度上具有对内毒素的拮抗作用和对肝线粒体膜的保护效应。

AIM： To investigate the effect of endotoxin on phospholipid of hepatic mitochondria membrane （MiM） and cation A （CA） antagonism in the model of endotoxemia in rabbits. METHODS： Forty- eight healthy Japanose big - ear rabbits were randomly divided into 3 groups： the normal control （ group Ⅰ ）, endotoxin treatment group （ group Ⅱ ） and cation A and endotoxin treatment group （ group Ⅲ ）. The contents of phosphatidylcholine （ PC）, phosphatidylethanolamin （PE）, phosphatidylserine （PS） and phosphatidylinositol （PI） in MiM of three groups were measured at the 3rd or 7th hour after corresponding treatment. RESULTS ： The contents of the four phospholipids in group Ⅱ were lower than those in group Ⅰ during the experiment （ P 〈 0. 01 ）. A significant increase in the contents of four phospholipids in group Ⅲ was observed as compared to group Ⅱ （ P 〈 0. 05, P 〈 0. 01 ）. CONCLUSION： Endotoxin decreases the major membrane phospholipids in MiM, whereas CA has a prominent protective effect on injuries by endotoxin in MiM and provides a valuable evidence for the use of some drugs of endotoxin antagonist.