丁酸钠诱导的未成熟树突状细胞的免疫学功能研究

Study on the immunological function of sodium butyrate-induced immature human monocyte-derived dendritic cells

目的:探讨丁酸钠对人外周血来源的树突状细胞(DC)的成熟状态和免疫学功能的影响。方法:通过粒细胞-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素4(IL-4)结合丁酸钠体外诱导人外周血来源的DC,6d后结合不同成熟因子诱导成熟,并以流式细胞仪、FITC标记的Dextran的内吞检测、混合淋巴细胞反应(MLR)、ELISA法分别检测DC的表面标志、内吞能力、DC刺激淋巴细胞增殖能力和白细胞介素12(IL-12)分泌量的改变。结果:丁酸钠可以抑制DC成熟,使DC具有较强的抗原吞噬能力,而刺激淋巴细胞增殖能力和IL-12的分泌能力下降。结论:丁酸钠可以抑制DC成熟,诱导不成熟DC生成,在移植免疫耐受方面具有较好的应用前景。

AIM： To investigate the immunological function of sodium butyrate - induced immature dendritic cells in vitro. METHODS ： The human monocyte - derived dendritic cells were induced in the presence of human granulocyte macrophage- colony stimulating factor（ GM -CSF） and interleukin- 4 （IL- 4）, combined with sodium butyrate. The immunological function of sodium butyrate - induced dendritic cells was detected by the FCM, endocytic activity, T cells stimulatory proliferation capacity, and interleukin - 12 （IL - 12） production. RESULTS： Sodium butyrate could down- regulate the major histocompatibility complex （MHC） class Ⅱ and costimulatory molecules of dendritic cells, increase the endocytic activity, induce a stage of T - cell anergey, and inhibit the T helper cell type 1 - skewing factor IL - 12 production. CONCLUSION： Sodium butyrate inhibits the maturation of dendritic cells and induces production of immature dendritic cells, which may help to explore the machenism of its epigenitic modification.