核酸适配体及其在化学领域的相关应用

Advance in Aptamer Research

核酸适配体是一小段经体外筛选得到的寡核苷酸序列,能与相应的配体进行高亲和力和强特异性的结合,它的出现为化学生物学界和生物医学界提供了一种新的高效快速识别的研究平台,并在许多方面展示了良好的应用前景。本文从核酸适配体的性质和体外筛选过程等方面出发,着重综述了核酸适配体的化学修饰方法及其在分析化学和酶化学中应用的研究进展。

Aptamer is a kind of synthetic oligonucleotides discriminated by in vitro screening, which is of high affinity and specificity with its ligands. Some new methods for efficient and fast detection for biochemical and biomedical purposes are being developed based on the molecular recognition of aptamers. In this paper, recent advance in the screening, chemical modification of aptamers and their applications in analytical chemistry and enzyme chemistry are reviewed.