外周神经损伤大鼠背根神经节中Ephrin B1及RYK受体表达的变化

Ephrin B1 and RYK expression in DRG of rats after peripheral nerve injury

目的研究外周神经损伤后背根神经节细胞中Ephrin B1及其相关受体的表达变化。方法建立一侧坐骨神经夹伤的大鼠动物模型,通过免疫荧光组织化学方法检测受损侧背根神经节细胞中Ephrin B1及其相关受体Eph B1、Eph B2、Eph B3和Eph A4、RYK等的表达,并分析阳性细胞数和不同大小阳性细胞的构成比例。结果外周坐骨神经受损侧背根神经节细胞中Ephrin B1的表达明显减弱,而Eph B1、Eph B2、Eph B3和Eph A4受体的表达无明显变化,但RYK受体的表达则明显加强。结论Ephrin B1和RYK受体在一侧外周坐骨神经夹伤后的大鼠背根神经节细胞中表达的变化,说明它很有可能参与了损伤后的功能活动。

Objective To study changes of expression in Ephrin and its receptors in DRG neurons after peripheral nerve injury. Methods A rat model of unilateral crushed sciatic nerve was prepared, and changes in the expression pattern of Ephrin B1, Eph B1 ,Eph B2 ,Eph B3 ,EphA4 and RYK in DRG neurons in the rats crushed unilateral sciatic nerve were estimated by the number of positive staining cells in DR（;, and the percentage of positive cells as well as the cell size. Results After peripheral nerve injury, the expression of Ephrin B1 significantly decreased, but there was no change in expression of Eph B 1, Eph B2, Eph B3 or Eph A4, RYK expression was intensely induced in DRG neurons. Conclusion Ephrin B1 and RYK showed very significant expression changes in DRG neurons after peripheral nerve injury, and may be a component factor process of peripheral never regeneration.