摘要
采用简单重复序列间扩增(ISSR)分子标记技术,对来自叶艺春兰同一母株并经多次无性繁殖获得的组培苗进行基因组DNA分析。研究结果表明,在多次继代培养过程中,组培苗遗传物质稳定,在DNA水平上未见明显差异,保持了母株的特性。
Inter-simple sequence repeats （ISSR） markers were used to investigate genetic stability for clonal plants of Cymbidium goeringji with verge line pattern leaves. The results showed that the culture clonal plants appeared without variations as compared with their original plants in substance of heredity and had no difference on the level of DNA.
出处
《亚热带植物科学》
2007年第2期13-14,共2页
Subtropical Plant Science
基金
湖北省自然科学基金(2004ABA119)资助项目
关键词
春兰
组培苗
ISSR
遗传稳定性
Cymbidium goeringii
seedling in vitro
ISSR
genetic stability
