鸡H-FABP基因多态性及其与肌内脂肪含量的相关研究

Study on Single Nucleotide Polymorphism of H-FABP Gene and Its Association with IMF in Chicken

根据GenBank发表的鸡H-FABP基因序列设计引物,以广东省农科院畜牧研究所提供的4个地方优质肉鸡品种,2个培育品种和1个引进的肉鸡品种为试验材料,通过测序和PCR-SSCP的方法进行SNP检测和基因型分析,探讨H-FABP基因多态性与肌内脂肪含量(IMF)之间的关系。结果发现在H-FABP基因260 bp和675 bp处存在T/C和G/A的突变位点,对2个突变位点在研究群体中进行基因型分析,结果产生3种基因型。基因型与IMF的相关分析结果表明:在T260C位点上,所有品种中AA基因型的IMF含量显著(P<0.05)高于BB基因型个体,但对于不同的品种来说,AA和BB基因型只对封开杏花鸡和岭南黄鸡II号的IMF含量有显著影响(P<0.05),而对其他各鸡种均无显著影响(P>0.05);在G675A位点上,所有品种中AA型和AB型肌内脂肪含量差异显著(P<0.05)。

In present study, four pairs of primer were designed according to the sequence of chicken H-FA BP gene offered by GenBank （Accession No：AY648562）.7 populations （including 4 native breeds, 2 breeds developed by Institute of Animal Science, Guangdong Academy of Agriculture Science and one foreign breed） were studied. All slaughter traits and meat quality traits were measured in the population. SNPs among individuals were detected by DNA sequencing, and PCR-SSCP method was then developed to screen the population. T/C and G/A mutation at the position of the 260 bp and 675 bp of H-FABP gene were found,and three genotypes were detected in the population. The least square showed that at the T260C locus in all population the AA birds had significant IMF value than BB birds （P 〈 0.05）, but AA and BB genotypes had significant effect on IMF analysis only in Fengkai Xinghua chicken and Lingnan Huang chicken population（P 〈 0.05）, but no differences among others （P 〉 0.05）. At the G675A locus in all populations, the AA birds had significant IMF value than AB birds （P 〈 0.05）.