补阳还五汤及不同配伍组方对缺血性脑中风后大鼠神经增殖作用的对比研究

Comparative Study of Buyang Huanwu Decoction and the Different Combinations of Its Ingredients on Neurogenesis Following Ischemic Stroke in Rats

目的观察补阳还五汤及不同配伍组方对缺血性脑中风后大鼠神经增殖作用的影响。方法采用机械开颅电凝法阻断大鼠大脑中动脉24h后,用随机电脉冲刺激仪每天刺激2h,连续15天,造成大鼠缺血性中风模型,分为模型组(给予等体积生理盐水灌胃)、补阳还五汤组、补阳还五汤去地龙组、当归补血汤组(同时给予相应药物灌胃进行干预治疗)。以免疫组织化学方法检测大鼠脑组织中5-溴脱氧核苷尿嘧啶(Br-dU)的表达。结果补阳还五汤组和补阳还五汤去地龙组大鼠海马区域中有大量BrdU阳性细胞存在,排列密集,部分呈簇状聚集,与模型组相比较,差异有显著性(P<0.01),并且脑缺血侧海马区域BrdU阳性细胞数与对侧比较显著增多(P<0.05);当归补血汤组与模型组和大鼠海马区域BrdU阳性细胞数比较,差异无显著性(P>0.05)。结论补阳还五汤促神经增殖作用优于当归补血汤。

Objective To investigate the effect of Buyang Huanwu Decoction （BYHWD） and the different combinations of its ingredients on neurogenesis following ischemic stroke in rats. Methods The model rats of ischemic stroke was established by blocking cerebral media artery with electrocoagulation through craniectomy, and electric stimulation, given from 24 h after blocking, 2 h daily for 15 successive days. They were divided into four groups, Group A treated with saline, Group B treated with BYHWD, Group C treated with BYHWD but earthworm subtracted, and Group D treated with Danggui Buxue Decoction （DGBXD）. The expression of 5- bromodeoxyuridine （BrdU） in cerebral tissue was determined by immunohistochemical method. Results Large amount of BrdU immunoreactive cells presented in the hippocampal region of rats in Group B and C, densely arranged, partial in cluster, with the figure significantly different to that in Group A （P〈0.01）, and the amount in the ischemic side was significantly more than that in the opposite side （P〈0.05）. While comparing between Group A and D, the amount of BrdU immunoreactive cells in the hippocampal region showed insignificant difference （P 〉0.05）. Conclusion BYHWD has a effect in promoting neurogenesis better than DGBXD.