血府逐瘀汤影响小鼠骨髓造血干细胞的实验研究

Experimental Study on Effect of Xuefu Zhuyu Decoction on Bone Marrow Hematopoietic Stem Cells of Mice

目的观察血府逐瘀汤对小鼠骨髓造血干细胞的数量、表型、细胞周期和集落形成能力的影响。方法昆明小鼠随机分为对照组及血府逐瘀汤高(13g/kg)、中(6.5g/kg)、低剂量(3.25g/kg)组,灌胃给药7天后,分离骨髓造血干细胞,进行细胞计数,流式细胞仪检测造血干细胞表型Sca和细胞周期,并采用甲基半固体培养基检测集落的形成。结果各组小鼠骨髓单核细胞和悬浮细胞数量以及集落产率差异无显著性(P>0.05),但低剂量组CD34和Sca-1的表达提高(P<0.05),中剂量组只能明显增加Sca-1的表达(P<0.05)。结论血府逐瘀汤通过增加较早期造血干细胞的数量和功能,提高造血能力,从而达到去瘀生新的作用。

Objective To investigate the effect of Xuefu Zhuyu Decoction （XFZYD） on the number, phenotype, cell cycle and colony formation of bone marrow hematopoietic stem cells （HSC） in mice. Methods Kunming mice were randomly divided into 4 groups： the control group, the low- （3.25 g/kg）, middle- （6.5 g/kg） and high-dose （13.0 g/kg） XFZYD groups. After they were medicated by gastrogavage respectively with saline or corresponding dose of XFZYD for 7 days, their bone marrow HSC were separated and counted. The phenotype Sca and cell cycle of HSC were detected by flow cytometer, and the colony formation was determined with semisolid methyl media culture. Results No obvious difference in the number of mononuclear cell, suspended cell and colony production was found among all the groups （P 〉 0.05） ; while the expression of CD34 and Sca-1 increased in the low-dose XFZYD group, but in the middle-dose XFZYD group increase only showed in Sca-1 expression. Conclusion XFZYD plays a role of removing blood stasis and promoting regeneration through improving hematopoietic function by means of increasing the number and enhancing the function of premature HSC.